HEPES Buffer CAS #7365-45-9

HEPES buffer(4 – (2-hydroxyethyl) – 1-piperazine ethanesulfonic acid) is a zwitterionic sulfonic acid buffer; One of good’s 20 buffers. Hepes buffer is widely used in cell culture, mainly because it can maintain physiological pH value better than bicarbonate buffer, which is also commonly used in cell culture, although the concentration of carbon dioxide (produced by aerobic respiration) changes. Lepe Zuniga et al. An unwanted photochemical process in which hepes buffer produces hydrogen peroxide when exposed to ambient light is reported, which is not a problem in bicarbonate based cell culture buffer. Therefore, it is strongly recommended to place the solution containing hepes buffer in the dark as far as possible to prevent oxidation.